Nobilis Rhino CV - Trials
- Protection trial - to determine length of immunity provided by Nobilis Rhino CV
- Compatability trials - to test the simultaneous use of Nobilis Rhino CV with certain other live vaccines.
Trial to determine length of immunity provided by Nobilis Rhino CV
Method
- Day old MDA* positive chicks vaccinated with Nobilis Rhino CV by coarse spray.
- Unvaccinated group as controls.
- 16 weeks post vaccination challenge with aMPV strain 11/94.
- Clinical signs recorded for 10 days post challenge.
Assessment of clinical signs
| Score | Clinical signs |
|---|---|
| 0 | No clinical signs |
| 1 | Slight nasal exudate |
| 2 | Moderate nasal exudate |
| 3 | Copius nasal exudate |
| 4 | Turbid nasal exudate |
| 5 | Ocular discharge |
| 6 | Swollen sinuses |
Results
Clinical signs following challenge with aMPV strain 11/94
Conclusion
Nobilis Rhino CV administered at one day of age in the presence of maternal antibodies provides good protection against APV infection for at least 16 weeks.
Compatibility of Nobilis Rhino CV with other live vaccines
Vaccines were administered by eye drop in various combinations. Challenges were done by eye drop and the birds were monitored for clinical signs.
Trial 1: Efficacy of Nobilis Rhino CV when given simultaneously with other live vaccines
| Day 1 | Day 7 | Day 14 | |
|---|---|---|---|
| Group 1 | Nobilis Rhino CV Nobilis H120 Nobilis NDC2 | Nobilis Gumboro D78 | Nobilis IB 4/91 |
| Group 2 | Nobilis H120 Nobilis NDC2 | Nobilis Gumboro D78 | Nobilis IB 4/91 |
| Group 3 | Nobilis Rhino CV Nobilis ND Clone 30 Nobilis IB Ma5 | Nobilis Gumboro D78 | Nobilis IB 4/91 |
| Group 4 | Nobilis Rhino CV | ||
| Group 5 | No vaccination | No vaccination | No vaccination |
Average clinical score per group
All groups were challenged with virulent aMPV strain 11/94 at 5 weeks of age by eye drop.
Trial 2: Efficacy of Newcastle Disease vaccination given simultaneously with Nobilis Rhino CV
| Vaccines given at day old | |
|---|---|
| Group 1* | Nobilis Rhino CV Nobilis ND Clone 30 |
| Group 2* | Nobilis Rhino CV Nobilis ND C2 |
| Group 3* | Nobilis ND C2 |
| Group 4* | No vaccination |
*20 chicks in each group
At 3 weeks old the birds were challenged with velogenic NDV intramuscularly.
Bird showing clinical signs of Newcastle disease 14 days post challenge
Trial 3: Efficacy of Infectious Bronchitis vaccination when given simultaneously with Nobilis Rhino CV
| Vaccines given at day old | |
|---|---|
| Group 1 | Nobilis IB H120 |
| Group 2 | Nobilis IB H120 Nobilis Rhino CV |
| Group 3 | Nobilis Rhino CV |
Three weeks later all 3 groups were challenged by eye drop with IB (Massachusetts) and protection was assessed using the ciliostasis test (the higher the score the fewer cilia are moving; a score of <20 indicates that the trachea is protected).
IB symptoms in each group after challenge
Serology
In all three trials Nobilis Rhino CV vaccination did not affect serum responses to any of the vaccines given.
Conclusion
The simultaneous administration of Nobilis Rhino CV with IB and ND vaccines gives full protection against Avian Pneumovirus infection and did not affect the performance of the tested vaccines.
